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Journal of Applied Physics / Volume 110 / Issue 12 / ARTICLES / Applied Biophysics

J. Appl. Phys. 110, 124701 (2011); http://dx.doi.org/10.1063/1.3669364 (6 pages)

Effects of cell cycle on the uptake of water soluble quantum dots by cells

Shen Zheng1, Ji-Yao Chen1, Jun-Yong Wang1, Lu-Wei Zhou1, and Qian Peng2

1State Key Laboratory of Surface Physics and Department of Physics, Fudan University, Shanghai 200433, People’s Republic of China
2Department of Pathology, Norwegian Radium Hospital, Oslo University Hospital, Montebello, Oslo 0310, Norway

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(Received 15 May 2011; accepted 13 November 2011; published online 19 December 2011)

Quantum dots (QDs) with excellent optical properties have become powerful candidates for cell imaging. Although numerous reports have studied the uptake of QDs by cells, little information exists on the effects of cell cycle on the cellular QD uptake. In this report, the effects of cell cycle on the uptake of water soluble thiol-capped CdTe QDs by the human cervical carcinoma Hela cell line, human hepatocellular carcinoma QGY7701 cell line, and human embryonic kidney 293T cell line were studied by means of laser scanning confocal microscopy and flow cytometry. All three cell lines show to take up CdTe QDs via endocytosis. After arresting cells at specific phases with pharmacological agents, the cells in G2/M phase take up the most CdTe QDs, probably due to an increased membrane expansion during mitosis; whereas the cells in G1 phase do the least. A mathematical physics model was built to calculate the relative uptake rates of CdTe QDs by cells in different phases of the cell cycle, with the result as the uptake rate in G2/M phase is 2-4 times higher than that in G1 phase for these three cell lines. The results obtained from this study may provide the information useful for intracellular delivery of QDs.

© 2011 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. MATERIALS AND METHODS
    1. Quantum dots
    2. Cell culture and QD treatments
    3. Cell imaging
    4. Cell synchronization
    5. Flow cytometric analysis
  3. RESULTS AND DISCUSSION
    1. Endocytosis responsible for cellular uptake of thiol capped QDs
    2. Effects of cell cycle on cellular uptake of QDs studied by LSCM
    3. Effects of cell cycle on cellular uptake of QDs studied by flow cytometry
  4. CONCLUSION

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KEYWORDS and PACS

Keywords

biomembranes, cadmium compounds, cellular biophysics, II-VI semiconductors, kidney, optical microscopy, physiological models, semiconductor quantum dots

PACS

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3669364

PUBLICATION DATA

ISSN

0021-8979 (print)  
1089-7550 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

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