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1 Oct 2010

Volume 108, Issue 7, Articles (07xxxx)

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J. Appl. Phys. 108, 071301 (2010); http://dx.doi.org/10.1063/1.3460809 (38 pages)

Y. H. Wu, T. Yu, and Z. X. Shen
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back to top Applied Biophysics

Auger electron spectroscopy for the determination of sex and age related Ca/P ratio at different bone sites

Ioannis Balatsoukas, Nikolaos Kourkoumelis, and Margaret Tzaphlidou

J. Appl. Phys. 108, 074701 (2010); http://dx.doi.org/10.1063/1.3490118 (6 pages) | Cited 1 time

Online Publication Date: 4 October 2010

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The Ca/P ratio of normal cortical and trabecular rat bone was measured by Auger electron spectroscopy (AES). Semiquantitative analysis was carried out using ratio techniques to draw conclusions on how age, sex and bone site affect the relative composition of calcium and phosphorus. Results show that Ca/P ratio is not sex dependent; quite the opposite, bone sites exhibit variations in elemental stoichiometry where femoral sections demonstrate higher Ca/P ratio than rear and front tibias. Age-related changes are more distinct for cortical bone in comparison with the trabecular bone. The latter’s Ca/P ratio remains unaffected from all the parameters under study. This study confirms that AES is able to successfully quantify bone mineral main elements when certain critical points, related to the experimental conditions, are addressed effectively.
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82.80.Pv Electron spectroscopy (X-ray photoelectron (XPS), Auger electron spectroscopy (AES), etc.)
87.64.ks Electron and photoelectron
61.66.Bi Elemental solids
61.66.Dk Alloys
87.64.-t Spectroscopic and microscopic techniques in biophysics and medical physics
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