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Journal of Applied Physics / Volume 107 / Issue 11 / ARTICLES / Applied Biophysics

J. Appl. Phys. 107, 114702 (2010); http://dx.doi.org/10.1063/1.3408446 (6 pages)

Surface profile dependence of the photon coupling efficiency and enhanced fluorescence in the grating-coupled surface plasmon resonance

Hironobu Hori1, Keiko Tawa1, Kenji Kintaka2, Junji Nishii2, and Yoshiro Tatsu1

1Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka 563-8577, Japan
2Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka 563-8577, Japan

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(Received 1 November 2009; accepted 26 March 2010; published online 4 June 2010)

The fluorescence excited by the enhanced electric field of grating-coupled surface plasmon resonance was detected on biochips. The enhancement of the electric field on a metallic grating surface was calculated using the finite difference time domain method for rectangular, trapezoidal, and sinusoidal models to clarify the grating-surface profile dependence of the enhanced fluorescence. The computational results showed that the enhancement in the trapezoidal model was stronger than that in the other models, and that the groove depth dependence of the enhancement in the trapezoidal model agreed well with the experimental data. The grating surface profile, including the groove depth, was found to be an important parameter of the grating structure for it to couple effectively with light.

© 2010 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. EXPERIMENTAL
    1. Fabrication of the substrates
    2. SPR-SPFS measurements
    3. Numerical analysis
  3. RESULTS AND DISCUSSION
    1. Fabrication of the substrates
    2. SPFS measurements
    3. FDTD calculations
      1. Distribution and groove-depth dependence of the surface-plasmon electric field
      2. Fluorescence quench effect for the enhanced fluorescence from a dye
      3. The surface profile dependence of the electric field intensity with resonance angle
  4. CONCLUSIONS

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KEYWORDS and PACS

Keywords

biological techniques, bio-optics, diffraction gratings, finite difference time-domain analysis, fluorescence, lab-on-a-chip, surface plasmon resonance

PACS

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3408446

PUBLICATION DATA

ISSN

0021-8979 (print)  
1089-7550 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    X. Cui, K. Tawa, H. Hori, and J. Nishii, Appl. Phys. Lett. 95, 133117 (2009)APPLAB000095000013133117000001.

    G. Lévêque and O. J. F. Martin, J. Appl. Phys. 100, 124301 (2006)JAPIAU000100000012124301000001.

    B. Fischer, T. M. Fischer, and W. Knoll, J. Appl. Phys. 75, 1577 (1994)JAPIAU000075000003001577000001.

    X. Luo and T. Ishihara, Appl. Phys. Lett. 84, 4780 (2004)APPLAB000084000023004780000001.


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